Figure 1: Acid fast staining of a fecal sample. Various bacteria and cryptosporidium could be identified.
Figure 2: Coccidia egg count being performed.
Figure 3: Isolation and cultivation of Campylobacter sp. for autogenic vaccine production.
A wide range of methods are employed for the detection, identification and enumeration of bacteria or parasites, ranging from biochemical to genetic based techniques. The starting point for the laboratory investigation is the correct field diagnosis of the diseased animal. The relevant clinical samples should then be collected and analyzed in the laboratory in order to isolate and identify the correct causative pathogen. Isolation of the disease causing pathogen allows for the production of autogenous vaccines and toxoid vaccines.
Establishing an Autogenous Mastitis or Blue udder vaccination program relies on the correct identification of bacterial organisms and their relation to previously isolated serotypes. These organisms are then propagated and used in the autogenous vaccine formulations.
Antibiogram evaluation is performed on cases were an immediate treatment is required. Here the identification of the most efficacious antibiotic can result in the quick and effective treatment of an animal.
Figure 4: Antibiogram evaluation of a bacteria isolated from a milk sample. Resistance toward certain antibiotics was identified, and the most effective antibiotic can be considered.